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The sequence parameters included a TE of 80 ms, TR of 2,000 ms, water suppression using a multiple optimized insensitive suppression train, pulse duration of 20 ms, an estimated water peak of 4.68 ppm, editing pulses interleaved at 1.9 ppm (ON) and 1.5 ppm (OFF), averaging two signals, a phase cycle of 16, frequency stabilization, and a total of 160 dynamic scans

Disruption of 12/15-lipoxygenase expression in peritoneal macrophages

The extract (100 L) made in ethanol and buffer was combined with 700 L of potassium phosphate buffer (pH 7.5, made of sodium phosphate dibasic heptahydrate and sodium phosphate monobasic monohydrate), 100 L of GSH, and 100 L of CDNB [25]

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This was confirmed by western blot analysis, which demonstrated a marked deficiency of the FAM134B protein in the renal cortex of these mice

Only one enzyme is known to breakdown GSH