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The amplified products were recombined and transformed on ice using DH5 competent cells, based on which the sequence for the plasmid was extracted and confirmed

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In some embodiments, the otic formulation or composition comprises about 0.01% by weight of the carbomer

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As anticipated, the ANOVA significant DIA sites were mainly upregulated in HeLa cells after 10 min EGF stimulation with an average log2 fold-change of 1.0 and belonging to the clusters B, C, and D of intermediate stimulators, late stimulators, and terminal effectors defined in the HeLa dataset 2