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Nickel beads are blue (left), but that color can change during protein purification. If the blue color doesnt return and your column is still white after elution, this means that you stripped the nickel ions off of the agarose beads, likely by using too much EDTA in your buffers

Naloxone, commonly known by the brand name Narcan, is a safe and effective medication that can temporarily reverse the effects of an opioid overdose, potentially saving lives

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