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In addition to the chemical methods mentioned above, enzymes such as peroxygenase and other epoxy alcohol synthases/hydroperoxide isomerases can be used to generate hydroxy-epoxides from hydroperoxides

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Activation of lactate receptor HCAR1 down-modulates neuronal activity in rodent and human brain tissue

BCA or Bradford protein assays were performed to determine protein concentrations, and the fractions were either used fresh or stored in aliquots at -80C (cellular membrane fractions) or -20C (cytosolic fractions)

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