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A total of 24 male Wistar rats were randomly divided into four groups with six rats in each group as follows: 9 9,10 Biochemical Analysis in Serum Rats were sacrificed by decapitation while under anesthesia on the fifth day, and carotid artery blood samples were collected into serum separation gel tubes

Methods Chemicals and enzymes 3-aminophthalhydrazide (luminol), 1-(p-hydroxyphenyl)imidazole (HPI), disodium sulfide (Na 2 S), erastin, RSL3, cumene hydroperoxide (CHP), liproxstatin-1, 7-(diethylamino)coumarin-3-carbohydrazide (CHH), 4-hydroxytamoxifen (Tam), monobromobimane (MBB), cysteine (Cys), 3-mercaptopyruvate (3MP), cytochrome c (cyt c), glutathione reductase (GR), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), S-nitrosoglutathione (GSNO), ferrocene, 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) and isopropyl -d-1-thiogalactopyranoside (IPTG) were obtained from Sigma-Aldrich

Since 1990, there has been a significant increase in mortality attributed to neurological diseases, with a rise of approximately 37% from 6.87 million to 9.40 million

Vajro P, Lenta S, Socha P, et al

GLS inhibition decreases the production of GSH in AML cell lines, leading to increased mitochondrial ROS and apoptosis [8, 24]

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