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Tailoring materials for modulation of macrophage fate

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This specific binding mode of optimized DEL7 analogs to the 3CL protease is different from all other current benchmark SARS-CoV-2 3CL protease non-covalent inhibitors, as demonstrated by the clear difference of 29 in a structural overlay of 3CL bound to it and other non-covalent inhibitors (Supplementary Fig

Macrophages (monocytes) are activated upon antigen stimulation and secrete various cytokines, such as TNF-, IL-18, IL-23, and IL-2

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After 4 days, the TS2/16 scFv-Fc was captured from the conditioned media with CaptivA HF protein-A affinity resin (Repligen, CA-PRI-0100) and eluted with IgG elution buffer (Thermo Scientific) containing 300 mM NaCl and neutralized with 100 mM Tris pH 8.0 to reach eventually pH 7.0