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Duranton, C., Huber, S
Subsequently, 10 L of CCK8 reagent (C0038, Beyotime) was added to each well, and incubation was continued for 2 h
The quality of the fractions was assessed using N-cadherin as a marker of membrane-enriched fractions and -actin as a marker of soluble (cytosol)-enriched fractions, which were also used as loading controls
Of the splice variants generated, only two long MLCK1 and MLCK2 are expressed in intestinal epithelial cells 157 (Fig
52 NealeREPurdieJLHirstLWGreenAC
Low ROS levels activate cell survival signaling pathways, such as the unfolded protein response and Nrf2 pathways, whereas high ROS levels activate cell death signaling pathways, such as apoptosis and necroptosis pathways 43
