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200 L tissue homogenate was mixed into a tube pre-added with 800 L of ice-cold methanol/water 80% (vol/vol) followed by vortexing rigorously for 1 min, and then centrifuge at ~ 20,160 g for 15 min in a refrigerated centrifuge

Then, the quantification result was corrected using a R package Accucor (v0.3.0) 47 for natural isotope correction

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Uddin, M.B., Lee, B.H., Nikapitiya, C., Kim, J.H., Kim, T.H., Lee, H.C., et al., J

55 At the molecular level, hair pigmentation is influenced by genes and signaling pathways such as Bmpr2, Acvr2a, and the Notch 1 and Notch 2 pathways