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Results Overall structures of CCK1R and CCK2R signaling complexes For structural studies with different transducer-coupled CCKRs (CCK1RG s and CCK2RG q ), human CCK1R and CCK2R with 15-residue depletion at the C-terminus were subcloned into pFastBac1 vector with an N-terminal Flag-tag, along with a LgBiT and double maltose-binding protein (MBP) affinity tag at the C-terminus to facilitate the protein expression and purification 42

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Given that highly invasive and metastatic cancer cells are more dependent on glutamine compared to less invasive cells (Yang et al ., 2014), combination therapy involving the inhibition of glutamine metabolism could constitute a novel approach to preventing metastasis
