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Subsequently, 500 L of ChIP dilution buffer (10 mm TrisHCl, pH 8, 150 mm NaCl, 1 mm EDTA, 1.1% Triton X-100, and cOmplete mini protease inhibitor-Roche 11836153001) was added to the lysate, and the mixture was centrifuged at 12,000 g for 10 min at 4 C

This matters because salt forms influence three critical properties: stability in acidic environments, solubility in biological fluids, and absorption through the gastrointestinal tract

We tell people to expect days, not minutes, and we measure rather than guess

However, it is important to note that not all symptoms resolve completely , especially if neurological damage was severe or prolonged before treatment commenced