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doi: 10.3389/fonc.2022.775649 Received 14 September 2021 Accepted 27 January 2022 Published 17 February 2022 Volume 12 - 2022 Edited by Bilian Jin, Dalian Medical University, China Reviewed by Tiziana Schioppa, University of Brescia, Italy

Strength: 50MG

Then neurons were washed three times in PBS for 5 min and mounted as described above

Injections bypass the digestive system, delivering B12 directly to the bloodstream

Different reconstitution volumes produce different concentrations from the same vial: 50mg vial + 2ml water = 25mg/ml (very concentrated) 50mg vial + 2.5ml water = 20mg/ml (concentrated) 50mg vial + 5ml water = 10mg/ml (standard) 50mg vial + 10ml water = 5mg/ml (dilute) Higher concentrations require smaller injection volumes but may exceed solubility limits for some peptides

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