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Relative quantification is achieved by comparing peptide spectrum matches (PSMs) from MS2 or extracted ion chromatography peak areas using MS1 intensity for a given protein across different conditions

[] The synthesis pathways of bile acid are remarkably similar in humans and mice, however, the contents of bile acids in bile acids pools are very different: the predominant primary synthesized bile acids in mice liver are CA (~50%) and - plus -muricholic acids (~50%), whereas the majority of primary bile acids in human liver are CA and CDCA

People at greatest risk for deficiency include pregnant women, who need more iron to make more blood to nourish a growing fetus

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