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17 Briefly, hepatocytes were isolated by digestion with type II collagenase (17101015, Gibco, Grand Island, USA) and then separated by Percoll (P1644, Sigma-Aldrich, USA) for centrifugation

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If glutathione stores are exhausted, however, the reactive intermediate combines with sulfhydryl groups on essential hepatic cell proteins, resulting in cell death

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Safety and efficacy of alpha lipoic acid during 4 years of observation: A retrospective, clinical trial in healthy subjects in primary prevention
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