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Samples were then transferred to 0.2 m centrifugal filters and centrifuged again at 10,000 RCF for 5 min at 4C before being transferred to glass auto-sampler vials ready for injection

LPL is secreted in an active and glycosylated from, but secretion is dependent on several critical steps that are facilitated by key molecular chaperones and binding partners

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Density increases from chylomicrons (CM, of lowest density) through lipoproteins of very low density (VLDL) , intermediate density (IDL) and low density (LDL), to high density lipoproteins (HDL)