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This finding is consistent with other reports that these genes expression typically peaks during early progenitor stage and decreases towards the final hepatocyte-like cells stage 24,25,26

The seeding medium consisted of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122)

However, side effects can occur

People with anxiety, stress and fatigue can feel lethargic, drowsy and unmotivated to do physical activities or eat properly, which then causes them to gain weight

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