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The spatial probe trial is used to test the subjects knowledge of the location of the platform which is demonstrated by the subject quickly swimming in the direction of the hidden platform

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Detection involved biotinylated secondary antibodies, streptavidin-peroxidase complex, and chromogen development with 3,3'-diaminobenzidine (DAB, Abcam), followed by hematoxylin counterstaining

Pinealon is a synthetic three-amino-acid peptide: glutamic acid, aspartic acid, and arginine (Glu-Asp-Arg), abbreviated EDR

For example, if you mix 10,000 IU of HCG with 10 mL of liquid, you get 1,000 IU per mL