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Principally, to achieve the desired loading efficiency, many key factors are documented

While the rate of ammonia fixation by the GDH reaction and the activity of the transaminases involved in the proposed shuttles may be sufficient to match the rate of glutamine synthesis, the modeling studies show that the actual fluxes of either the alanine/lactate or the BCAA/branched chain keto-acid shuttles are insufficient to account for the need of ammonia nitrogen to account for glutamine synthesis

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Improved production of Humira antibody in the genetically engineered Escherichia coli SHuffle, by co-expression of human PDI-GPx7 fusions

doi: 10.1111/cas.14490 66 SteggerdaSMBennettMKChenJ