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Before the experiment, a working solution of dihydroxyrhodamine (5 M) is diluted from the stock, kept on ice, and protected from light
Rinninella E, Cintoni M, Raoul P, et al
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The cells were washed twice with PBS, 400 l of PBS were added to each well, and fluorescence intensity was determined with a flow cytometer (analyzed by FlowJo 7.6.1)

10.1007/s12035-019-1474-9 210 ShindeR

Extremophiles 11:445452
