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frozen transfers: Frozen embryo transfers may result in higher initial beta hCG levels than fresh transfers One study found these median beta hCG levels (13 days after embryo transfer) among pregnancies that progressed to a clinical pregnancy: Fresh Day 3: 400 mIU/mL Frozen Day 3: 600 mIU/mL Frozen Day 5 (blastocyst): 937 mIU/mL Embryo stage at transfer: Blastocyst (Day 5) transfers typically yield higher and earlier hCG levels than cleavage-stage (Day 3) embryos due to earlier implantation