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Oxygen removal stock solution was prepared by mixing 2 mL of 400 mM Tris-HCl solution with pH 7.5, 2 mL of 100 mM KCl solution, 2 mL of 4 mM TCEP with pH 7, 2 mL of 20 mg mL -1 glucose oxidase solution and 2 mL of 1.6 mg mL 1 catalase solution, followed by adding 10 mL of glycerol

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The output of the computer analysis of the primary sequence takes the form of a graph (Figure 02-22), where the x-axis is the linear sequence of the protein, listed from N-terminus to C-terminus, and the y-axis is a number known as the hydropathy index
