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Protocol 1: moderate weight loss (10-15% body weight) Goal: Steady, sustainable weight loss with minimal side effects Tirzepatide approach: Standard escalation to 5-7.5 mg Hold at the dose where you achieve consistent weekly loss of 0.5-1% body weight Expected timeline: 24-36 weeks to target Likely maintenance dose: 5-7.5 mg Retatrutide approach: Standard escalation to 4 mg 4 mg delivered 12.9% weight loss at 24 weeks in trials Hold at 4 mg if achieving target rate Expected timeline: 20-30 weeks to target For moderate goals, both compounds work well at their lower dose ranges

(Princeton, NJ, USA) using a validated liquid chromatography electrospray ionisation with tandem mass spectrometric detection method over the concentration ranges of 102000 and 1000200 000 ng ml 1 (Latz et al, 2006)

-hydroxybutyrate as biomarker of hepatic fatty-acid oxidation