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TUNEL assay A TUNEL Kits (Servicebio, Wuhan, G1501) was used as directed by the manufacturer

FLS were first identified by HE and anti-vimentin immunostaining (Fig

The presence of colchicine, chlorpromazine, and indomethacin significantly reduced the cellular uptake of SC-NEs, suggesting that micropinocytosis-, clathrin- and caveolae-mediated endocytosis pathways were involved in the uptake process

Pathophysiologic processes and novel biomarkers associated with congestion in heart failure

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This binding activates receptor autophosphorylation, which triggers a downstream signaling cascade through the phosphorylation of tyrosine residues of the insulin receptor substrates, IRS (IRS-1 or IRS-2), followed by phosphorylation of phosphatidylinositol 3-kinase (PI3K), phosphoinositide dependent kinase-1, Akt (Akt1 and Akt2), protein kinase C (PKC) and mammalian target of rapamycin (mTOR), as well as ribosomal protein S6 kinase beta 1 (S6K1) [10, 11]