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While freezing doesnt chemically destroy benzyl alcohol, the freeze-thaw cycle can cause the preservative to separate from the water
For researchers exploring multiple options, understanding these differences helps in designing rational protocols

[DOI] [PMC free article] [PubMed] [Google Scholar] 55.Cluntun A.A., Lukey M.J., Cerione R.A., Locasale J.W

Cell viability is generally reduced by freeze-thawing in any cell type

Mann, J., Emslie, G., Baldessarini, R

New York, NY, USA: 2000
