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39 The outcome of these studies reinforces the notion that the intestinal epithelium is essential for separating luminal antigens from mucosal immune components, and that its disruption by altered proliferation/apoptosis ratio can result in immune activation and inflammation (Figure 1)

The ability of proliferation was assessed by a Cell Counting Kit-8 (CCK-8, Dojindo, Rockville, MD, United States) at different time points post-plating

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The concurrent reduction in synaptic plasticity and cognitive performance further supports that HDAC9 is required for maintaining synaptic plasticity and cognitive function