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710 Cuproptosis represents a metal-dependent RCD defined in 2022, in which intracellular copper binds to lipoylated tricarboxylic-acid (TCA) cycle enzymes, most notably dihydrolipoamide S-acetyltransferase (DLAT), driving protein oligomerisation/aggregation, loss of FeS-cluster proteins, and mitochondrial proteotoxic stress in cells reliant on oxidative phosphorylation

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Nevertheless, to study the modulation of this concentration under particular physiological or pathological conditions, the subtraction of GSSG is needed

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This preserves nicotinamide for NAD production, improving energy and fat-burning efficiency