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The data were corrected for carbon isotope effects and chain length, and analyzed using in-house-developed software (RALP)

Subsequently, the KFERQ-like sequence for protein targeting to lysosomes was identified (31), and it was confirmed that heat shock cognate 70 (HSC70) binds to this region, targeting substrates to lysosomes for ATP-dependent degradation, initiating research into chaperone recognition for selective lysosomal degradation (32)