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Plates were washed and treated with alkaline phosphatase-conjugated anti-mouse secondary antibodies (BioRad) at a 1:3000 ratio for 1 hour

This personalized measurement prevents both intramuscular injection (too long) and intradermal administration (too short), optimizing medication absorption and reducing injection-site reactions or discomfort

Switch from cyanocobalamin to methylcobalamin (or vice versa), drop the dose, or change the delivery route

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