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For stable cell lines, GFP was cloned into pLJM1 vector between AgeI and EcoRI sites and assembled in Stbl3 competent cells by In-Fusion ligation (Takara Bio)

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If we add to this 20.3% corresponding to the heterozygous 677CT/WT, the 677 isoform is responsible for more than 80% of the cases with elevated Hcy

10.2353/ajpath.2008.080119 [DOI] [PMC free article] [PubMed] [Google Scholar] Griess B., Tom E., Domann F., Teoh-Fitzgerald M

Releasing fascial tissue within the body and at the occipital base (where skull and top of neck meet) allows for optimal restriction release and fluid exchange prior to cranial holds