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The column was washed with 25 mM Tris (pH 7.5), 200 mM NaCl and 10 mM imidazole, and eluted with 25 mM Tris (pH 7.5), 200 mM NaCl and 500 mM imidazole
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Studies have shown that SCFAs not only regulate energy homeostasis by influencing insulin sensitivity and glucose utilization in the host (Rios-Covian et al., 2017)
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Systemic considerations GHK-Cu is well tolerated systemically
doi: 10.1016/j.ejps.2010.06.010
