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Stress exposure, food intake and emotional state

The societal costs of diabetic neuropathy are outnumbered only by the individual costs to each patient, including pain, inability to work, poor QOL, multiple hospitalizations for ulcers and eventual amputations

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doi:10.1523/JNEUROSCI.20-24-08972.2000 Tretter L, Adam-Vizi V (2004) Generation of reactive oxygen species in the reaction catalyzed by alpha-ketoglutarate dehydrogenase

Genomic DNA was isolated

After desalting thrice with 400 L of 80% EtOH, the magnetic beads were subjected to enzymatic digestion in 100 L of 5 ng/L Trypsin Gold (Promega) and 50 mM AMBC for 3.5 h at 37 C and rinsed out with 100 L of pure water