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Glutamate is produced intracellularly from glutamine via glutaminase, which supplies glutamate for both xCT activity and for tricarboxylic (TCA) cycle anaplerosis

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For example, the soluble cytosolic coumarate 3-hydroxylase (C3H) enzymes of A

The protein bands were acquired through ECL chemiluminescence and quantified and standardized using ImageJ software v1.8.0