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Membranes were blocked in 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 1 h at room temperature and incubated overnight with primary antibodies at dilutions specified in Supplementary Table 26

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Neurological signs include numbness, trouble walking, and balance issues

In addition to reducing or eliminating the symptoms of B12 deficiency, research suggests that B12 shots may: Reduce stress (7) Support central nervous system function, red blood cell formation, and DNA synthesis (2) Reduce the risk of vision loss from age-related macular degeneration (8) Lower homocysteine levels, which is associated with cardiovascular disease and stroke (9, 10)
