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Always store refrigerated B12 injections within the recommended temperature range and avoid exposing them to freezing conditions

First, all the rats were anesthetized via an intraperitoneal injection of 2% pentobarbital sodium (75 mg/kg), and the hair of the right posterior leg was removed

These skin changes reflect diminished circulation and hypoxia potentiating capillary occlusion

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The effect is particularly noticeable in poorly vascularized tissues like tendons, which normally heal slowly due to limited blood flow