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The selected ALCAR concentrations are in line with our previously results on endothelial cells, and other preclinical and clinical studies [17, 20, 24, 30, 36]

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Brains were homogenized using lysis buffer for whole protein and mitochondrial protein extraction as previously described (Nguyen et al., Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes

images preparationF.G.M., S.M