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It is derived from two other amino acids, lysine and methionine, and can be synthesized by the body or obtained through diet, mainly from animal-based foods such as red meat and dairy products

Protein preparation and Western blot analysis Cultured cells or flashfrozen tissues were homogenized in icecold radioimmunoprecipitation assay (RIPA) buffer (50 mM TrisHCl pH 7.6, 150 mM NaCl, 0.5% sodium deoxycholate, 1% NP40, 0.1% SDS, 2 mM EDTA) containing COMPLETE protease inhibitor cocktail (Roche) and phosphatase inhibitors (Santa Cruz Biotechnology)

R., Abel, G., Sidhar, S

Among these compounds, GHK-Cu remains one of the most widely studied due to its relationship with cellular signaling, tissue maintenance, and regenerative pathways

10.1007/s12265-023-10470-x 289 ZhaoX.TanF.CaoX.CaoZ.LiB.ShenZ.et al (2020)