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However, an excessive ROS level, commonly referred to as oxidative stress, induces damage to deoxyribonucleic acid (DNA), proteins, and lipids, ultimately culminating in cell death [3, 6]

Cells were washed with cold PBS and total proteins were extracted with RIPA buffer containing Halt inhibitors (Thermofisher, MA)

The formation of structural disulfide bonds in proteins, for instance, is a tightly organized process catalyzed by specialized enzymes like oxidoreductases and thiol oxidases [64] and occurs mostly in dedicated cellular compartments, such as the periplasmic space, the endoplasmic reticulum (ER) and the mitochondrial intermembrane space (IMS) [6466]

2013;2(6):e000542