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Description
Studies were carried out on adult or neonatal mice and rats

Insulin-like Growth Factor I Levels in Proportionate Dogs, Chondrodystrophic Dogs and in Giant Dogs

For immunohistochemistry (IHC) staining, the right hemisphere of each brain was collected and fixed in 4% paraformaldehyde

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Cells were washed and fixed with methanol (100%) and permeabilized with Triton X-100 (0.5%) in PBS

Hydrogen can also enter the mitochondria and nucleus
