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Samples for analysis were fractionated via Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Fisher Scientific) according to Manufacturers instructions

doi: 10.2337/diab.40.12.1615

Up to 20% of people over 60 may become deficient according to UCLA Health (2022), and the National Institutes of Health estimates that 10 to 30% of older adults cannot absorb naturally occurring vitamin B12 from food