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WT and Slc7a11-KD tumor inoculation To generate pLKO.1-Slc7a11shRNA plasmid, forward oligonucleotide (5-CCGGGCCCTGTCCTATGCAGAATTACTCGAGTAATTCTGCATAGGACAGGGCTTTTTG-3) was annealed with reverse oligonucleotide (5-AATTCAAAAAGCCCTGTCCTATGCAGAATTACTCGAGTAATTCTGCATAGGACAGGGC-3), and then subcloned into an AgeI (#R0552S, NEB, USA)/EcoRI (#R0101S, NEB, USA) digested pLKO.1-TRC cloning vector (#10879, Addgene, USA)

The primary site of expression of the DBH gene is in the adrenal glands

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However, this increase in IL production requires further investigation

Walle A, Andreeff M , Melamed MR