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In the meantime, the clinical studies that we have performed with broccoli and broccoli sprouts have already strained the academic system to the breaking point [142]

mRNA levels were quantified using a Fast SYBR Green Master Mix (Applied Biosystems) on a 7900HT Fast Real time PCR System (Applied Biosystems)

Petrosino, S

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8.10 Biochemical effects Biochemical effects have been observed in cultured hepatocytes, liver homogenates and mitochondrial fractions, purified enzymes, isolated hepatic lipid membranes and whole liver preparations, including: metabolic effects on lipid peroxidation (Stege, 1982