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Peptide purification, TMT-labeling, and high-pH basic fractionation Peptides were desalted on a 50 mg Sep-Pak C18 Vac (Waters Corporation) using a vacuum manifold

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Real-time polymerase chain reaction (PCR) was performed by using a kit (SYBR Premix EX Taq, Takara Bio Inc.) and the ABI PRISM 7300 real-time PCR system