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The respiratory quotient, the ratio of carbon dioxide released to the amount of oxygen consumed, indicates substrate utilization rates

doi: 10.1007/s12020-012-9751-7

For this, PBMC were isolated by density gradient centrifugation (Biocoll 1.077 g/ml, Biochrom AG, Berlin, Germany) from heparinized blood samples

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This highlights a critical gap in clinical practice, emphasizing the need for hepatologists and gastroenterologists to be more proactive in identifying smoking as a risk factor for liver disease progression and in implementing strategies to promote smoking cessation among their patients