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For the perfusions, mice were deeply anesthetized with isoflurane and perfused transcardially with saline, followed by a 10% buffered formalin solution (150200 mL per mouse)

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This is potentially due to the sample containing a sub-fraction of mis-folded protein or the samples being partially refolded

Through the exosomeglialinflammation cascade, this mechanism facilitates the spread of focal neuronal injury to adjacent brain regions, thereby accelerating region-specific neurodegeneration and functional impairment in Alzheimers disease (Figure 3)

When administered under licensed medical supervision using pharmaceutical-grade compounds, the combination has a well-documented safety profile