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Expression and purification of recombinant APEX2 APEX2 was expressed from a plasmid (72558 pTRC-APEX2, Addgene) in Escherichia coli BL21(DE3) (EC0114, Thermo Fisher Scientific), as described previously 23
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However, the long-term effects of vitamin C pretreatment on cisplatin-induced mechanical and cold allodynia were lowered paw withdrawal thresholds, consistent with the development of cisplatin-induced allodynia, without significant differences [91]
doi: 10.1104/pp.110.161794 194 TianL.LiJ.BiW.ZuoS.LiL.LiW.et al
