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Seventy percent of melanoma arises de novo and is not associated with a precursor melanocytic nevus

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Cbh was expressed and purified through immobilised metal ion affinity chromatography (IMAC) with nickel-charged agarose resins followed by size exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (GE Healthcare Life Sciences)

(PDF 1707 kb) (PDF 164 kb) Rights and permissions About this article Cite this article Fernandes, S., Salta, S., Bravo, J
