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Metal-Polyphenol-Network Coated Prussian Blue Nanoparticles for Synergistic Ferroptosis and Apoptosis via Triggered GPX4 Inhibition and Concurrent In Situ Bleomycin Toxification

IGF axis activity associates with deregulated immune genes and immune infiltrating cells in PCa tissues To test the clinical relevance of these findings we interrogated public RNA-seq data from the Prostate Adenocarcinoma Firehose Legacy dataset in cbioportal (www.cbioportal.org/study/summary?id=prad_tcga) 30

Cancer organoids from mice were cultured in tumour organoid medium (CM) composed of Advanced DMEM/F12 (Gibco, 12634) supplemented with 1% penicillinstreptomycin solution, 0.1% BSA, 2 mM l-glutamine, 10 mM Hepes, 50 ng/mL EGF (PeproTech AF-100-15), 100 ng/mL Noggin (PeproTech 250-38), 500 ng/mL Spondin (PeproTech 120-38), 1X N-2 Supplement (ThermoFisher 17502048) and 1X B-27 Supplement (ThermoFisher 17504044)

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This is critical, as GSH is essential for T cell effector functions through its regulation of metabolic activity (Mak et al., 2017)
