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For saponification, cell pellets were mixed with 15% (w/v) NaOH in 50% (v/v) methanol (1 ml), incubated at 100C for 5 min, vortexed and further incubated for 25 min

(a) ABHD5 interaction to ATGL was predicted using Interactome INSIDER ( [46]) and the involved residues were depicted on the ABHD5 3D model (ModBase B2R9K0, ribbon representation with key residues as space-filling spheres)

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This explains why people with T1D still face a twofold higher cardiovascular risk compared to the general population,despite major progress in glucose control

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