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Although most immobilization techniques involve coupling ligands through amine or sulfhydryl groups, the large number of such groups can create a problem with improper orientation or multipoint attachment

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Permeabilization was performed using 0.5% Triton X-100 (Sigma) for 5 min at room temperature

Among these, the complex mechanisms of phenotypic transformation of astrocytes and microglia in CVD represent an important direction for future research

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DOI:10.1016/j