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A deficiency is characterised by megaloblastic anaemia, fatigue, weakness, constipation, loss of appetite, and weight loss

Face: The most responsive area, with the strongest clinical evidence

Water volume added: The exact amount of bacteriostatic water you used

The libraries were sequenced as 100 bp paired-end reads on a NovaSeq 6000 system (Illumina)

Thus, overall, more work needs to be done to determine whether manipulating NAD + levels is effective in reversing ageing-related immunological dysfunction in the adaptive immune system and, equally important, whether such manipulations are safe