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The tissue was homogenized thrice at 1500 rpm for 120 s using a tissue homogenizer (Micro Smash MS100R, Tomy Digital Biology Co., Ltd., Tokyo, Japan) following which the homogenate was centrifuged at 2300 g and 4 C for 5 min

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5.1 Relationship between migraine and oxidative stress 5.1.1 Clinical evidence of oxidative stress 5.1.1.1 Antioxidant capacity In contrast to the control group, patients with migraine without aura had higher total oxidative status (TOS), higher oxidative stress index (OSI), and lower total antioxidant status (TAS) (Tripathi et al., 2018)

Compounding this vulnerability, the immature gastrointestinal tract of preterm infants faces specific risks

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